| dc.description.abstract | Crinum jagus is a medicinal plant used traditionally to treat tuberculosis, malaria and other
bacterial infections. However, there are limited documented scientific studies to substantiate the
use of this plant. Due to increase in resistance to malaria and tuberculosis drugs, the need for the
development of other drugs is pertinent. This study was designed to determine the
pharmacological activities of extract and fractions of Crinum jagus.
Methanol extract of C. jagus obtained by soxhlet extraction was subjected to phytochemical
analysis and fractionated using column chromatography. Antitubercular and antimicrobial
activities of the extract and its fractions were evaluated against isolates of Mycobacterium
tuberculosis and selected microorganisms using the disc and agar diffusion methods.
Antimalarial activity was assessed in vivo using Rane’s test in Plasmodium berghei infected
mice (n = 80 in 10 groups) treated orally with tween 80 (control), 10, 25, 50 and 75 mg/kg of
extract and its fractions at 10 mg/kg respectively, while chloroquine (10 mg/kg) and arteether (3
mg/kg) groups served as positive controls. Anti-inflammatory potential was assessed in rats
using carrageenan-induced paw inflammatory model. In vitro antioxidant potentials were
determined spectrophotometrically using 1,1-diphenyl-2-picryl hydrazyl (DPPH), hydroxyl
radical scavenging activities, Total Flavonoids Contents (TFC) and Phenolic Contents (TPC)
Antioxidant indices- Superoxide dismutase (SOD) and Catalase (CAT) activities and levels of
Malondialdehyde (MDA) and reduced Glutathione (GSH) were determined by
spectrophotometry. Aspartate (AST) and Alanine (ALT) amino transferases and Alkaline
Phosphatase (ALP) were estimated spectrophotometrically. Data were analysed by Student’s t
test at p = 0.05.
Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenols and steroids in
the crude extract. The extract and its fractions (F1, F2 and F3) showed a concentrationdependent
inhibition of Mycobacterium tuberculosis, with F1 having the lowest IC50of :
0.22mg/mL relative to rifampicin (IC50 : 0.19mg/mL) and isoniazid (0.23mg/mL). The extract at
10, 25, 50, 75 mg/kg and F1, F2 and F3 at 10 mg/kg suppressed parasitaemia in Plasmodium
berghei infected mice by 70.0, 76.0, 79.0, 87.0% and 89.0, 76.0, 78.0% respectively relative to
chloroquine (100%) and arteether (89.0%). The extract at 10, 25, 50, 75 mg/kg and F1, F2 and
F3 at 10 mg/kg inhibited oedema in rat paws by 26.0, 30.0, 32.0, 66.0% and 80.0, 25.0, 52.0% iii
respectively when compared with indomethacin (95.0%). The extract and its fractions
significantly scavenged DPPH and hydroxyl radical in vitro. The TPC and TFC of extract, F1, F2
and F3 at 500 μg/ml were 0.310, 0.460, 0.240, 0.380 μg/mg and 0.523, 0.864, 0.396, 0.643 μg/g
respectively. The extract and its fractions significantly reduced MDA level while GSH, SOD and
CAT levels were increased. Activities of AST, ALT and ALP were significantly increased at 50
and 75 mg/kg body weight of extract .
Crinum jagus exhibited antitubercular, antimalarial and anti-inflammatory activities via
scavenging of radicals and antioxidative mechanism. This indicates a promising potential of the
plant for drug development. | en_US |
